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Ampligen inhibits human herpesvirus-6 in vitro.

Authors
  • Ablashi, D V1
  • Berneman, Z N
  • Williams, M
  • Strayer, D R
  • Kramarsky, B
  • Suhadolnik, R J
  • Reichenbach, N
  • Hiltzges, P
  • Komaroff, A L
  • 1 National Cancer Institute, Bethesda, MD 20892.
Type
Published Article
Journal
In vivo (Athens, Greece)
Publication Date
Jan 01, 1994
Volume
8
Issue
4
Pages
587–591
Identifiers
PMID: 7893986
Source
Medline
License
Unknown

Abstract

The recently discovered human herpesvirus-6 (HHV-6) is being associated with an increasing number of conditions in which there is evidence of immunologic dysfunction. A number of widely available antiviral agents have shown little or no activity against the virus. We found that Ampligen [Poly (1): Poly (C12U), a synthetic, mismatched, double-stranded RNA, has potent, previously unexpected antiviral effects. Cells known to allow replication of HHV-6 were infected with the virus and treated with Ampligen under various conditions. When cells were pretreated with Ampligen (concentrations of 100 or 200 micrograms/ml) prior to infection or treated shortly after infection, viral replication was inhibited by 46-98%. At 100 and 200 micrograms/ml, Ampligen also inhibited the DNA polymerase activity of HHV-6 by 42-98%. When lower concentrations of Ampligen (10 and 50 micrograms/ml) were used, only pretreatment of cells, with Ampligen, followed by virus infection and carrying the infected cells with Ampligen, significantly inhibited HHV-6 infection (83.7 and 89.1% respectively). Indirect evidence suggests that Ampligen may inhibit viral attachment to cellular receptors and/or inhibit intracellular maturation of the virus. The above concentrations of Ampligen were not toxic to the cells used in the study. Given these in vitro findings, and the low frequency of toxicity reported with the use of Ampligen, clinical trials of this drug in patients with evidence of reactivated HHV-6 infection would seem to be warranted.

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