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Amplification of six putative RD1 genes of Mycobacterium tuberculosis for cloning and expression in Escherichia coli and purification of expressed proteins.

Authors
  • Amoudy, Hanady A
  • Mustafa, Abu S
Type
Published Article
Journal
Medical principles and practice : international journal of the Kuwait University, Health Science Centre
Publication Date
Jan 01, 2008
Volume
17
Issue
5
Pages
378–384
Identifiers
DOI: 10.1159/000141501
PMID: 18685277
Source
Medline
License
Unknown

Abstract

All of the six targeted RD1 genes were amplified and five expressed using E. coli hosts, but only two of the expressed proteins were purified to homogeneity. Alternative expression systems are required to obtain all RD1 proteins for functional characterization.

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