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Amplification of fluorescence detection of DNA based on magnetic separation.

Authors
  • Nie, Li-bo
  • Wang, Xiu-li
  • Li, Song
  • Chen, Hong
Type
Published Article
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Publication Date
Nov 01, 2009
Volume
25
Issue
11
Pages
1327–1331
Identifiers
PMID: 19907090
Source
Medline
License
Unknown

Abstract

A novel nanoparticles-based fluorescence detection method has been developed by taking advantage of magnetic separation and amplified fluorescence detection. This DNA sensor relies on a "sandwich" hybridization strategy, in which the DNA targets are first hybridized to captured oligonucleotide probes immobilized on magnetic nanoparticles, and then hybridized with thiol-modified oligonucleotide probes immobilized on gold nanoparticles. Subsequently, the amplified DNA signals are detected in the form of bio-bar-code DNA using a chip-based fluorescence detection method. The result showed that the detection limit of target DNA probes is 1 pM. Complementary and mismatched sequences were clearly distinguished, and the ratio of the background-subtracted fluorescence values for complementary and single-base mismatched oligonucleotide was 2.12:1. This new system can be applied to both DNA detection and immunoassay, and has broad potential applications in disease diagnosis and immunoassay.

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