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Amidrazone modified acrylic fabric activated with cyanuric chloride: A novel and efficient support for horseradish peroxidase immobilization and phenol removal.

Authors
  • Almulaiky, Yaaser Q1
  • El-Shishtawy, Reda M2
  • Aldhahri, Musab3
  • Mohamed, Saleh A4
  • Afifi, Mohamed5
  • Abdulaal, Wesam H6
  • Mahyoub, Jazem A7
  • 1 Chemistry Department, Faculty of Sciences and Arts, University of Jeddah, Khulais, P.O. Box 355, Khulais 21921, Saudi Arabia; Chemistry Department, Faculty of Applied Science, Taiz University, Taiz, Yemen. Electronic address: [email protected] , (Saudi Arabia)
  • 2 Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia; Dyeing, Printing and Textile Auxiliaries Department, Textile Research Division, National Research Center, Dokki, 71516, Cairo, Egypt. , (Egypt)
  • 3 Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia; Center of Nanotechnology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. , (Saudi Arabia)
  • 4 Molecular Biology Department, National Research Centre, Cairo, Egypt. , (Egypt)
  • 5 Department of Biochemistry, Faculty of Science, University of Jeddah, P.O. Box 80203, Jeddah 21589, Saudi Arabia; Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, Egypt. , (Egypt)
  • 6 Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia. , (Saudi Arabia)
  • 7 Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia. , (Saudi Arabia)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Nov 01, 2019
Volume
140
Pages
949–958
Identifiers
DOI: 10.1016/j.ijbiomac.2019.08.179
PMID: 31445147
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this study, hydrazine treated acrylic fabrics (polyacrylonitrile, PAN) activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The immobilization of HRP onto the modified supporting material was achieved after being end-over-end incubated for 12 h. Field emission scanning electron microscopy and Fourier-transform infrared spectroscopy techniques were used to confirm the successful immobilization. Reusability experiment was performed to estimate the ability of the immobilized HRP to recover the reaction medium, in which it was observed to retain 78% of its original activity after 10 cycles. Relative to the soluble HRP, the optimum pH and temperature for the immobilized HRP were shifted to 7-7.5 and 50 °C, respectively. The kinetic parameters of guaiacol and H2O2 for the immobilized HRP were determined to be Km/Vmax = 57.61, 11.35 and Kcat/Km = 1.87, 1.86, respectively, while the values for the free form were Km/Vmax = 41.49, 6.23 and Kcat/Km = 1.87, 1.86, respectively. Compared to the soluble form, the immobilized HRP exhibited higher resistance toward metal ions and some organic solvents. For an application perspective. The immobilization of HRP using this procedure has the potential to be used for industrial application and wastewater treatment. Copyright © 2019 Elsevier B.V. All rights reserved.

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