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Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene Expression, and Behavioral Patterns in Rats' Obesity-Induced Diabetes

  • Elnagar, Asmaa1
  • El-Dawy, Khalifa1
  • El-Belbasi, Hussein I.1
  • Rehan, Ibrahim F.2
  • Embark, Hamdy3
  • Al-Amgad, Zeinab4
  • Shanab, Obeid5
  • Mickdam, Elsayed6
  • Batiha, Gaber E.7
  • Alamery, Salman8
  • Fouad, Samer S.9
  • Cavalu, Simona10
  • Youssef, Mohammed3
  • 1 Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig , (Egypt)
  • 2 Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Menofia University, Shebin Alkom , (Egypt)
  • 3 Department of Physiology, Faculty of Veterinary Medicine, South Valley University, Qena , (Egypt)
  • 4 General Authority for Veterinary Services, Ph.D in Veterinary Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena , (Egypt)
  • 5 Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena , (Egypt)
  • 6 Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, South Valley University, Qena , (Egypt)
  • 7 Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour , (Egypt)
  • 8 Department of Biochemistry, College of Science, King Saud University, Riyadh , (Saudi Arabia)
  • 9 Qena University Hospital, Ph.D in Veterinary Clinical Pathology, South Valley University, Qena , (Egypt)
  • 10 Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, Oradea , (Romania)
Published Article
Frontiers in Public Health
Frontiers Media SA
Publication Date
Apr 07, 2022
DOI: 10.3389/fpubh.2022.777129
  • Public Health
  • Original Research


Amelioration of hyperinsulinemia and insulin resistance associated with obesity is a cardinal target for therapeutics. Therefore, we investigated the relation of Fibrilln-1 (FBN1) mRNA expression and hepatic phosphoenolpyruvate caboxykinase (PEPCK) enzyme to the ameliorative impact of oxytocin on obesity-induced diabetes, suggesting glycogenolysis markers in diabetic models. Four groups of forty male Wistar rats were formed (n = 10): a control group fed basal diet and intraperitoneal injections of saline; an oxytocin-injected group; a diet-induced obese group fed a high-fat/high-sugar diet and injected with saline; a diet-induced obese group injected with oxytocin. Depending on blood glucose levels, obese groups were further sub-grouped into prediabetic, and diabetic rats, with 5 rats each, at the ninth and the 16th week of the feeding period, respectively. FBN1 expression and PEPCK activity were determined using the qPCR technique and some biochemical parameters (glycemic, lipid profile, kidney, and liver functions) were determined using kits. Obese groups showed an elevation of brain FBN1 expression, high serum lipid profile, high glucose level, and a deleterious impact on liver and kidney functions. Obese groups showed the stimulator effect of the PEPCK enzyme and time-dependent pathological changes in renal and hepatic tissues. The motor activities were negatively correlated with FBN1 gene expression in prediabetic and diabetic rats. In addition to our previous review of the crucial role of asprosin, here we showed that oxytocin could ameliorate obesity-induced diabetes and decrease FBN1 gene expression centrally to block appetite. Oxytocin caused decreases in PEPCK enzyme activity as well as glycogenolysis in the liver. Therefore, oxytocin has a potential effect on FBN1 expression and PEPCK enzyme activity in the obesity-induced diabetic-rat model.

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