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Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction

Authors
  • Wang, Xue1
  • Cao, Yongge2
  • Liu, Zhenhua1
  • Wang, Zihan3
  • Chu, Xiaoying1
  • Wang, Lei4
  • Hu, Xuanxuan1
  • Zhao, Han4
  • Diao, Zhanqiu3
  • Peng, Fengting3
  • Ye, Hui1
  • Cao, Zhensheng3
  • 1 Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China , (China)
  • 2 Haiyuan College, Kunming Medical University, Kunming, Yunnan, China , (China)
  • 3 School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China , (China)
  • 4 School of the Second Clinical Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China , (China)
Type
Published Article
Journal
Balkan medical journal
Publication Date
Oct 28, 2019
Volume
36
Issue
6
Pages
311–319
Identifiers
DOI: 10.4274/balkanmedj.galenos.2019.2018.12.11
PMID: 31290639
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Oral breathing can cause morphological changes in the oral and maxillofacial regions. To investigate whether oral breathing affected structural changes in bone tissues. Animal experimentation. A total of 48 8-day-old male Sprague−Dawley rats were divided into two groups: a breathing group and a sham (control) group. All Sprague−Dawley rats were killed at 7 weeks after unilateral nostril obstruction modeling. Then, structural changes in bone tissues were detected by micro-computed tomography, and the expression levels of receptor activator of nuclear factor-κB, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in the signal pathway of bone metabolism within the local alveolar bone and serum of rats were detected by reverse transcription-quantitative polymerase chain reaction and Western blotting. The results showed that receptor activator of nuclear factor-κB ligand and receptor activator of nuclear factor-κB levels in bone tissues and serum in the oral breathing group were higher than those in the control group [Maxillary alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.009, pprotein=0.008), receptor activator of nuclear factor-κB (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.047, pprotein=0.042), receptor activator of nuclear factor-κB (pRNA=0.041, pprotein=0.007); Serum: receptor activator of nuclear factor-κB ligand (pRNA<0.001, pprotein<0.001), receptor activator of nuclear factor-κB (pRNA<0.001, pprotein<0.001)], along with decreased osteoprotegerin expression (Maxillary alveolar bone: pRNA=0.038, pprotein=0.048; Mandibular alveolar bone: pRNA<0.001, pprotein<0.001; Serum: pRNA=0.009, pprotein=0.006) and elevated receptor activator of nuclear factor-κB ligand/osteoprotegerin. Micro-computed tomography analysis indicated a significant difference in the level of bone volume fraction, as well as trabecular thickness in maxillary alveolar bone between the experimental and control groups (p=0.049, p=0.047). Meanwhile, trabecular thickness, and cortical thickness levels in mandibular alveolar bone also differed significantly between the experimental and control groups (p=0.043, p=0.024). Structural changes of the respiratory system affect the alveolar bone structure and unilateral nasal obstruction may lead to a change in regional specific bone density.

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