Affordable Access

Publisher Website

Altrenogest during early pregnancy modulates uterine glandular epithelium and endometrial growth factor expression at the time implantation in pigs

  • Muro, Bruno Bracco Donatelli1
  • Leal, Diego Feitosa1
  • Carnevale, Rafaella Fernandes1
  • Torres, Mariana Andrade1
  • Mendonça, Maitê Vidal1
  • Nakasone, Denis Hideki1
  • Martinez, Cristian Hernando Garcia1
  • Ravagnani, Gisele Mouro1
  • Monteiro, Matheus Saliba1
  • Poor, André Pegoraro1
  • Martins, Simone Maria Massami Kitamura2
  • Viau, Priscila1
  • de Oliveira, Cláudio Alvarenga1
  • de Castro, Raquel Vasconcelos Guimarães3
  • Bessi, Brendon Willian3
  • Bressan, Fabiana Fernandes3
  • Pulz, Lidia Hildebrand3
  • Strefezzi, Ricardo Francisco3
  • Almond, Glen William4
  • de Andrade, André Furugen Cesar1
  • 1 Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, SP, Brasil
  • 2 Departamento de Zootecnia, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil
  • 3 Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil
  • 4 College of Veterinary Medicine, North Carolina State University, North Carolina, Raleigh, United States of America
Published Article
Animal Reproduction
Colégio Brasileiro de Reprodução Animal
Publication Date
May 28, 2021
DOI: 10.1590/1984-3143-AR2020-0431
PMID: 34122654
PMCID: PMC8189350
PubMed Central
  • Original Article


This study evaluated the effects of supplying altrenogest from day 6-12 of pregnancy on the endometrial glandular epithelium, corpora lutea (CL) morphology, and endometrial and CL gene expression. A total of 12 crossbred females (Landrace × Large White) were used. The females were assigned to 4 treatments according to a random design with a 2 × 2 factorial arrangement, with two categories (sow or gilt) and two treatments (non-treated and treated with altrenogest). On day 6 of pregnancy, animals were allocated to one of the following groups: non-treated (NT, n = 6; 3 sows and 3 gilts), and (T, n = 6; 3 sows and 3 gilts) treated daily with 20 mg of altrenogest, from day 6-12 of pregnancy. All animals were euthanized on day 13 of pregnancy. All CLs were individually weighed, and their volume were determined. The endometrial glandular density (GD), mean glandular area (MGA), and vascular density (VD) were determined by histomorphometric and immunohistochemical analyses. Endometrium samples were collected and analyzed by qRT-PCR to evaluate the abundance of transcripts for VEGF and IGF-I. Females in the T group had higher MGA ( P < 0.05) compared to the NT group. There was no effect of treatment on GD or VD for both experimental groups. Sows in the T group had augmented expression of IGF-I ( P < 0.05). Progestagen had no detrimental effect on CL morphology. In conclusion, altrenogest improves the uterine environment during the peri-implantation period in pigs without compromising corpora lutea development.

Report this publication


Seen <100 times