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Alternative Polyadenylation: Methods, Findings, and Impacts.

Authors
  • Chen, Wei1
  • Jia, Qi1
  • Song, Yifan1
  • Fu, Haihui1
  • Wei, Gang2
  • Ni, Ting3
  • 1 State Key Laboratory of Genetic Engineering & MOE Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai 200438, China. , (China)
  • 2 State Key Laboratory of Genetic Engineering & MOE Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai 200438, China. Electronic address: [email protected] , (China)
  • 3 State Key Laboratory of Genetic Engineering & MOE Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai 200438, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Genomics, proteomics & bioinformatics
Publication Date
Oct 01, 2017
Volume
15
Issue
5
Pages
287–300
Identifiers
DOI: 10.1016/j.gpb.2017.06.001
PMID: 29031844
Source
Medline
Keywords
License
Unknown

Abstract

Alternative polyadenylation (APA), a phenomenon that RNA molecules with different 3' ends originate from distinct polyadenylation sites of a single gene, is emerging as a mechanism widely used to regulate gene expression. In the present review, we first summarized various methods prevalently adopted in APA study, mainly focused on the next-generation sequencing (NGS)-based techniques specially designed for APA identification, the related bioinformatics methods, and the strategies for APA study in single cells. Then we summarized the main findings and advances so far based on these methods, including the preferences of alternative polyA (pA) site, the biological processes involved, and the corresponding consequences. We especially categorized the APA changes discovered so far and discussed their potential functions under given conditions, along with the possible underlying molecular mechanisms. With more in-depth studies on extensive samples, more signatures and functions of APA will be revealed, and its diverse roles will gradually heave in sight.

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