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Altered Genome-Wide Methylation in Endometriosis

Authors
  • Naqvi, Hanyia1
  • Ilagan, Ysabel1
  • Krikun, Graciela1
  • Taylor, Hugh S.1
  • 1 Yale University, 333 Cedar Street, New Haven, CT, 06520, USA , New Haven (United States)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Oct 01, 2014
Volume
21
Issue
10
Pages
1237–1243
Identifiers
DOI: 10.1177/1933719114532841
Source
Springer Nature
Keywords
License
Yellow

Abstract

Endometriosis has been associated with aberrant methylation in the eutopic endometrium. Using a genome-wide methylation array, we identified differentially methylated genes in the endometrium from women with or without endometriosis. One hundred and twenty genes were significantly altered by >1.5-fold. In all, 59 genes were significantly hypermethylated and 61 genes were significantly hypomethylated. Changes in gene expression associated with the altered methylation status were validated using quantitative real-time polymerase chain reaction. A limited number of candidate genes are selectively methylated in the endometrium of women with endometriosis. Several genes not previously associated with endometriosis are aberrantly methylated and expressed. These include O-6-methylguanine-DNA methyltransferase, dual specificity phosphatase 22, cell division cycle associated 2, inhibitor of DNA binding 2, retinoblastoma binding protein 7, bone morphogenetic protein receptor, type 1B, tumor necrosis factor receptor 1B, zinc finger protein receptor 681, immunoglobulin superfamily, member 21, and tumor protein 73. Aberrant DNA methylation and gene expression of these genes may contribute to abnormal regulation of endometrial cell proliferation and function in women.

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