Affordable Access

Alterations in mitogen-induced calcium mobilization and intracellular free calcium produced by 7,12-dimethylbenz(a)anthracene in the Jurkat human T cell line.

Authors
  • Burchiel, S W1
  • Thompson, T A
  • Davis, D A
  • 1 University of New Mexico, College of Pharmacy Toxicology Program, Albuquerque 87131. , (Mexico)
Type
Published Article
Journal
International Journal of Immunopharmacology
Publisher
Elsevier
Publication Date
Jan 01, 1991
Volume
13
Issue
1
Pages
109–115
Identifiers
PMID: 1902825
Source
Medline
License
Unknown

Abstract

The purpose of these studies was to assess the effects of 7,12-dimethylbenz(a)anthracene (DMBA), an immunosuppressive polycyclic aromatic hydrocarbon (PAH), on Ca+2 mobilization induced by phytohemagglutinin (PHA) in the Jurkat human T cell line. Intracellular levels of free cytosolic Ca+2 were examined by flow cytometry using Indo-1 loaded cells. At doses of 3-30 microM, DMBA was found to produce a dose and time-dependent inhibition of Ca+2 mobilization in Jurkat following in vitro exposure. The decrease in Ca+2 mobilization was correlated with an increase in baseline levels of cytoplasmic free Ca+2. Two non-immunosuppressive PAH, benzo(e)pyrene and anthracene, failed to inhibit PHA-induced Ca+2 mobilization or alter baseline levels of free Ca+2. These results suggest that DMBA may produce immunosuppression by inhibiting Ca+2 mobilization or by altering Ca+2 homeostasis in activated T cells.

Report this publication

Statistics

Seen <100 times