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Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen.

Authors
  • Collins, Matthew D
  • Higgins, Robert
  • Messier, Serge
  • Fortin, Madeleine
  • Hutson, Roger A
  • Lawson, Paul A
  • Falsen, Enevold
Type
Published Article
Journal
International journal of systematic and evolutionary microbiology
Publication Date
May 01, 2003
Volume
53
Issue
Pt 3
Pages
811–814
Identifiers
PMID: 12807205
Source
Medline
License
Unknown

Abstract

An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on L-lysine and a DNA G + C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to Alkalibacterium olivapovliticus, Alloiococcus otitis, Dolosigranulum pigrum and related organisms, in the low-G + C-content Gram-positive bacteria. However, sequence divergence values of > 11% from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, Allofustis seminis gen. nov., sp. nov. The type strain is strain 01-570-1(T) (= CCUG 45438(T) = CIP 107425(T)).

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