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All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines.

Authors
  • Chen, Hongjun
  • Angel, Matthew
  • Li, Weizhong
  • Finch, Courtney
  • Gonzalez, Ana Silvia
  • Sutton, Troy
  • Santos, Jefferson
  • Perez, Daniel R
Type
Published Article
Journal
Journal of Virology
Publisher
American Society for Microbiology
Publication Date
Sep 01, 2014
Volume
88
Issue
17
Pages
10013–10025
Identifiers
DOI: 10.1128/JVI.01468-14
PMID: 24942589
Source
Medline
License
Unknown

Abstract

Vaccination is the first line of defense against influenza virus infections. A major drawback in the preparation of influenza vaccines is that production relies on a heavily time-consuming process of growing the viruses in eggs. We propose a radical change in the way influenza vaccination is approached, in which a recombinant bacmid, a shuttle vector that can be propagated in both Escherichia coli and insect cells, carries an influenza virus infectious clone (bcmd-RGFlu). Using a surrogate cell system, we found that intranasal delivery of bcmd-RGFlu resulted in generation of influenza virus in mice. Furthermore, mice vaccinated with this system were protected against lethal influenza virus challenge. The study serves as a proof of principle of a potentially universal vaccine platform against influenza virus and other pathogens.

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