Affordable Access

Publisher Website

Alginate-waterborne polyurethane 3D bioprinted scaffolds for articular cartilage tissue engineering.

Authors
  • Olmos-Juste, R1
  • Larrañaga-Jaurrieta, G2
  • Larraza, I1
  • Ramos-Diez, S3
  • Camarero-Espinosa, S4
  • Gabilondo, N5
  • Eceiza, A6
  • 1 'Materials + Technologies' Research Group (GMT), Department of Chemical and Environmental Engineering, Engineering College of Gipuzkoa, University of the Basque Country UPV / EHU, Plaza Europa 1, Donostia / San Sebastian 20018, Gipuzkoa, Spain. , (Spain)
  • 2 POLYMAT, University of the Basque Country UPV / EHU, Avenida Tolosa 72, Donostia / San Sebastián 20018, Gipuzkoa, Spain; Regenerative Medicine Lab, CICbiomaGUNE, Donostia / San Sebastián 20014, Gipuzkoa, Spain. , (Spain)
  • 3 POLYMAT, University of the Basque Country UPV / EHU, Avenida Tolosa 72, Donostia / San Sebastián 20018, Gipuzkoa, Spain. , (Spain)
  • 4 POLYMAT, University of the Basque Country UPV / EHU, Avenida Tolosa 72, Donostia / San Sebastián 20018, Gipuzkoa, Spain; Ikerbasque, Basque Foundation for Science, Euskadi Pl., 5, 48009, Bilbao, Spain. , (Spain)
  • 5 'Materials + Technologies' Research Group (GMT), Department of Chemical and Environmental Engineering, Engineering College of Gipuzkoa, University of the Basque Country UPV / EHU, Plaza Europa 1, Donostia / San Sebastian 20018, Gipuzkoa, Spain. Electronic address: [email protected]. , (Spain)
  • 6 'Materials + Technologies' Research Group (GMT), Department of Chemical and Environmental Engineering, Engineering College of Gipuzkoa, University of the Basque Country UPV / EHU, Plaza Europa 1, Donostia / San Sebastian 20018, Gipuzkoa, Spain. Electronic address: [email protected]. , (Spain)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Dec 31, 2023
Volume
253
Issue
Pt 4
Pages
127070–127070
Identifiers
DOI: 10.1016/j.ijbiomac.2023.127070
PMID: 37748588
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Articular cartilage defects comprise a spectrum of diseases associated with degeneration or damage of the connective tissue present in particular joints, presenting progressive osteoarthritis if left untreated. In vitro tissue regeneration is an innovative treatment for articular cartilage injuries that is attracting not only clinical attention, but also great interest in the development of novel biomaterials, since this procedure involves the formation of a neotissue with the help of material support. In this work, functional alginate and waterborne polyurethane (WBPU) scaffolds have been developed for articular cartilage regeneration using 3D bioprinting technology. The particular properties of alginate-WBPU blends, like mechanical strength, elasticity and moistening, mimic the original cartilage tissue characteristics, being ideal for this application. To fabricate the scaffolds, mature chondrocytes were loaded into different alginate-WBPU inks with rheological properties suitable for 3D bioprinting. Bioinks with high alginate content showed better 3D printing performance, as well as structural integrity and cell viability, being most suitable for scaffolds fabrication. After 28 days of in vitro cartilage formation experiments, scaffolds containing 3.2 and 6.4 % alginate resulted in the maintenance of cell number in the range of 104 chondrocytes/scaffold in differentiated phenotypes, capable of synthesizing specialized extracellular matrix (ECM) up to 6 μg of glycosaminoglycans (GAG) and thus, showing a potential application of these scaffolds for in vitro regeneration of articular cartilage tissue. Copyright © 2023. Published by Elsevier B.V.

Report this publication

Statistics

Seen <100 times