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Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectors.

Authors
  • De Kathen, A
  • Jacobsen, H J
Type
Published Article
Journal
Plant cell reports
Publication Date
Sep 01, 1990
Volume
9
Issue
5
Pages
276–279
Identifiers
DOI: 10.1007/BF00232301
PMID: 24226826
Source
Medline
License
Unknown

Abstract

Epicotyl segments and nodus expiants from etiolated seedlings of Pisum sativum were transformed using Agrobacterium tumefaciens strains GV 2260 (p35S GUS INT) and GV 3850 HPT carrying either a neomycin- or hygromycinphosphotransferase-gene as selectable markers. The transgenic character of hygromycin- or kananamycin-resistant tissue was confirmed by detection of nopaline or neomycinphosphotransferase-II- and ß-glucuronidase activity in crude extracts of resistant tissues. Up to 5 % of developing shoots from shoot proliferating nodi were regenerated via organogenesis to kanamycin-resistant plantlets. Transformation frequency in vitro was found to be influenced by expiant source, A. tumefaciens strain, pea genotype and duration of cocultivation. Acetosyringone did not increase the transformation rate.

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