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Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant

Authors
  • Suvorov, Alexander1
  • Pilsner, J. Richard
  • Naumov, Vladimir
  • Shtratnikova, Victoria1
  • Zheludkevich, Anna
  • Gerasimov, Evgeny2
  • Logacheva, Maria1, 3
  • Sergeyev, Oleg1, 4
  • 1 (O.S.)
  • 2 Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia
  • 3 Center for Life Sciences, Skolkovo Institute of Science and Technology, 143028 Moscow, Russia
  • 4 Chapaevsk Medical Association, Meditsinskaya Str. 3a, Samara Region, 446100 Chapaevsk, Russia
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Nov 04, 2020
Volume
21
Issue
21
Identifiers
DOI: 10.3390/ijms21218252
PMID: 33158036
PMCID: PMC7672616
Source
PubMed Central
Keywords
License
Green

Abstract

Advanced paternal age at fertilization is a risk factor for multiple disorders in offspring and may be linked to age-related epigenetic changes in the father’s sperm. An understanding of aging-related epigenetic changes in sperm and environmental factors that modify such changes is needed. Here, we characterize changes in sperm small non-coding RNA (sncRNA) between young pubertal and mature rats. We also analyze the modification of these changes by exposure to environmental xenobiotic 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47). sncRNA libraries prepared from epididymal spermatozoa were sequenced and analyzed using DESeq 2. The distribution of small RNA fractions changed with age, with fractions mapping to rRNA and lncRNA decreasing and fractions mapping to tRNA and miRNA increasing. In total, 249 miRNA, 908 piRNA and 227 tRNA-derived RNA were differentially expressed (twofold change, false discovery rate (FDR) p ≤ 0.05) between age groups in control animals. Differentially expressed miRNA and piRNA were enriched for protein-coding targets involved in development and metabolism, while piRNA were enriched for long terminal repeat (LTR) targets. BDE-47 accelerated age-dependent changes in sncRNA in younger animals, decelerated these changes in older animals and increased the variance in expression of all sncRNA. Our results indicate that the natural aging process has profound effects on sperm sncRNA profiles and this effect may be modified by environmental exposure.

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