Aggrecan: Approaches to Study Biophysical and Biomechanical Properties.
Department of Biomedical Engineering, Boston University, Boston, MA, USA.
Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Department of Biological Engineering, Electrical Engineering and Computer Science, and Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, UK. [email protected].
- Published Article
Methods in molecular biology
Clifton, N.J. : Humana Press
- Publication Date
Jan 01, 2022
Aggrecan, the most abundant extracellular proteoglycan in cartilage (~35% by dry weight), plays a key role in the biophysical and biomechanical properties of cartilage. Here, we review several approaches based on atomic force microscopy (AFM) to probe the physical, mechanical, and structural properties of aggrecan at the molecular level. These approaches probe the response of aggrecan over a wide time (frequency) scale, ranging from equilibrium to impact dynamic loading. Experimental and theoretical methods are described for the investigation of electrostatic and fluid-solid interactions that are key mechanisms underlying the biomechanical and physicochemical functions of aggrecan. Using AFM-based imaging and nanoindentation, ultrastructural features of aggrecan are related to its mechanical properties, based on aggrecans harvested from human vs bovine, immature vs mature, and healthy vs osteoarthritic cartilage. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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This record was last updated on 06/06/2022 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/34626381