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Aged garlic extract induces proliferation and ameliorates gentamicin-induced toxicity in LLC-PK1 cells.

Authors
  • Velasco-Velázquez, Marco A
  • Maldonado, Perla D
  • Barrera, Diana
  • Torres, Víctor
  • Zentella-Dehesa, Alejandro
  • Pedraza-Chaverrí, José
Type
Published Article
Journal
Phytotherapy research : PTR
Publication Date
Jan 01, 2006
Volume
20
Issue
1
Pages
76–78
Identifiers
PMID: 16397848
Source
Medline
License
Unknown

Abstract

Gentamicin (GM)-induced nephrotoxicity limits the use of this antibiotic. It has been shown that aged garlic extract (AGE) and S-allylcysteine (SAC), the most abundant organosulfur compound in AGE, ameliorate GM-induced nephrotoxicity in rats. The present communication evaluated the effect of AGE and SAC on proliferation and on GM-induced toxicity and genotoxicity of porcine kidney epithelial cell line (LLC-PK1 cells). The cells were preincubated with different concentrations of AGE or SAC for 12 h before incubation with 8 mm GM for an additional 72 h. At the end of this time, cell viability, genotoxicity and proliferation were evaluated. AGE stimulated cell proliferation and protected LLC-PK1 cells from GM-mediated toxicity and genotoxicity. SAC partially prevented only GM-induced genotoxicity. These results suggest that the stimulation of cell proliferation could possibly be one of the mechanisms involved in the in vitro protective effect of AGE in GM-induced toxicity of LLC-PK1 cells.

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