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Age-associated changes in GSH S-transferase gene/proteins in livers of rats

Authors
  • Xu, Shangfu1, 2
  • Hou, Dongshun1
  • Liu, Jie1
  • Ji, Lili2
  • 1 Key Lab for Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine, Zunyi Medical University, People’s Republic of China
  • 2 Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, People’s Republic of China
Type
Published Article
Journal
Redox Report : Communications in Free Radical Research
Publisher
Taylor & Francis
Publication Date
Nov 16, 2018
Volume
23
Issue
1
Pages
213–218
Identifiers
DOI: 10.1080/13510002.2018.1546985
PMID: 30444463
PMCID: PMC6748684
Source
PubMed Central
Keywords
License
Green

Abstract

Objectives : Glutathione S-transferases (GSTs) are phase-II metabolic enzymes playing important roles in drug metabolism, anti-oxidative stress and anti-aging. Age is a key factor influencing GSTs expression. Thus, age-related changes of 10 GSTs were examined. Methods : Livers from male Sprague–Dawley rats at fetus (−2 d), neonates (1, 7, 14 and 21 d), puberty (28 and 35 d), adulthood (60 and 180 d), and aging (540 and 800 d), were collected and subjected to qPCR analysis. Liver proteins from 14, 28, 60, 180, 540 and 800 d were also extracted for selected protein analysis by Western-blot. Results : The expression of GSTA1 and GSTP1 increased over the life span and the expression of GSTA4, GSTO1 and GSTZ1 gradually increased until adulthood, and slightly decreased at 800 days. The expression of GSTM1, GSTM3, GSTT1, GSTT2 and GSTK1 gradually increased until adulthood, but significantly decreased during aging of 540 and 800 days. There is a small peak at 7–14 d for GSTA1, GSTP1 and GSTZ1. The protein expression of GSTA1, GSTM1 and GSTP1 followed the trend of mRNA changes. Discussion : This study characterized three expression patterns of 10 GSTs during development and aging in rat liver, adding to our understanding of anti-aging role of GSTs.

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