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Age-dependent transcription factor (ADF) interacts with prostate-specific antigen (PSA) gene enhancer.

Authors
  • Zaidi, Ghazala
  • Supakar, Prakash C
Type
Published Article
Journal
Molecular biology reports
Publication Date
Sep 01, 2002
Volume
29
Issue
3
Pages
325–328
Identifiers
PMID: 12463425
Source
Medline
License
Unknown

Abstract

Human prostate-specific antigen (PSA) is clinically most useful diagnostic marker for prostate cancer. The PSA gene is partially regulated by androgen hormone via androgen receptor (AR). Several transcription factors including novel transcriptional regulator, age-dependent factor (ADF) bind to AR promoter and play role in the regulation of AR gene expression. Earlier, an androgen responsive enhancer (-5824 to -3738) has been identified in 5'-flanking region of PSA gene. Here, we demonstrate by competitive electrophoretic mobility shift assay that ADF binds to a 19 bp sequence in PSA gene (-4372 to -4390) located within this enhancer region. This suggests that ADF may play a role in the regulation of PSA gene expression.

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