An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells.
- Published Article
- Publication Date
Dec 01, 1978
Agarose disc gel electrophoresis has been adapted to achieve the separation of the major sulfated glycosaminoglycans produced by cells in culture. By use of buffers containing barium ion, mixtures of chondroitin sulfate, dermatan sulfate, and heparan sulfate are well resolved into discrete bands. The technique can be used preparatively as well as analytically to separate quantities of glycosaminoglycans up to a milligram in a 6-mm diameter gel.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/9762132