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An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells.

Authors
  • Funderburgh, J L
  • Chandler, J W
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Dec 01, 1978
Volume
91
Issue
2
Pages
464–472
Identifiers
PMID: 9762132
Source
Medline
License
Unknown

Abstract

Agarose disc gel electrophoresis has been adapted to achieve the separation of the major sulfated glycosaminoglycans produced by cells in culture. By use of buffers containing barium ion, mixtures of chondroitin sulfate, dermatan sulfate, and heparan sulfate are well resolved into discrete bands. The technique can be used preparatively as well as analytically to separate quantities of glycosaminoglycans up to a milligram in a 6-mm diameter gel.

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