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AFM and Microrheology in the Zebrafish Embryo Yolk Cell.

Authors
  • Marsal, Maria1
  • Jorba, Ignasi2
  • Rebollo, Elena1
  • Luque, Tomas2
  • Navajas, Daniel2
  • Martín-Blanco, Enrique3
  • 1 Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas.
  • 2 Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias.
  • 3 Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas; [email protected]
Type
Published Article
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Publication Date
Nov 29, 2017
Issue
129
Identifiers
DOI: 10.3791/56224
PMID: 29286426
Source
Medline
Language
English
License
Unknown

Abstract

Elucidating the factors that direct the spatio-temporal organization of evolving tissues is one of the primary purposes in the study of development. Various propositions claim to have been important contributions to the understanding of the mechanical properties of cells and tissues in their spatiotemporal organization in different developmental and morphogenetic processes. However, due to the lack of reliable and accessible tools to measure material properties and tensional parameters in vivo, validating these hypotheses has been difficult. Here we present methods employing atomic force microscopy (AFM) and particle tracking with the aim of quantifying the mechanical properties of the intact zebrafish embryo yolk cell during epiboly. Epiboly is an early conserved developmental process whose study is facilitated by the transparency of the embryo. These methods are simple to implement, reliable, and widely applicable since they overcome intrusive interventions that could affect tissue mechanics. A simple strategy was applied for the mounting of specimens, AFM recording, and nanoparticle injections and tracking. This approach makes these methods easily adaptable to other developmental times or organisms.

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