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Alterations in the baseline sister-chromatid exchange frequency in human lymphocyte culture following a number of cell divisions

Authors
Journal
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
0027-5107
Publisher
Elsevier
Publication Date
Volume
127
Issue
2
Identifiers
DOI: 10.1016/0027-5107(84)90016-2
Disciplines
  • Ecology
  • Geography

Abstract

Abstract A significant decrease in the baseline of sister-chromatid exchanges (SCEs) was observed in cultured human lymphocytes, if 5-bromodeoxyuridine (BrdU) was added after 60 h of culture, and the cells were harvested at least 24–30 h after BrdU exposure. This decrease is supposed to occur if at least one cell division takes place before the addition of BrdU. For cytogenetic monitoring of mutagenic environmental factors, using human lymphocyte cultures, it is assumed that two time periods are sufficient for comparison.

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