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Preparative isolation of vitamin D2from previtamin D2by recycle high-performance liquid chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
590
Issue
1
Identifiers
DOI: 10.1016/0021-9673(92)87019-5
Disciplines
  • Economics

Abstract

Abstract A preparative high-performance liquid chromatographic method, based on recycle chromatography, to separate vitamin D 2 (ergocalciferol) from previtamin D is described. The method provides efficient separation by means of a mixture of methanol, acetonitrile and hexane as eluent on a reversed-phase C 18 column. Scale-up to a 2-in. diameter column resulted in the collection of 100% pure fractions based on UV detection at 265 nm. The total throughput and the economics of the purification were also optimized.

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