Abstract The electrochemical behavior of Acebutolol was examined by cyclic voltammetry and coulometry. The products of Acebutolol oxidation were then characterized by liquid chromatography (LC) and mass spectrometry (MS). An aromatic ether cleavage reaction was observed and various intermediate species were identified. In the presence of glutathione, a series of glutathione adducts were identified enabling possible oxidation pathways of Acebutolol to be established. The obtained results are useful for predicting and understanding the oxidative Acebutolol degradation pathway.