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Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.).

Authors
  • Dong, H T1
  • Techatanakitarnan, C1, 2
  • Jindakittikul, P1
  • Thaiprayoon, A1
  • Taengphu, S2
  • Charoensapsri, W2, 3
  • Khunrae, P1
  • Rattanarojpong, T1
  • Senapin, S2, 3
  • 1 Department Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok, Thailand. , (Thailand)
  • 2 Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand. , (Thailand)
  • 3 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand. , (Thailand)
Type
Published Article
Journal
Journal of fish diseases
Publication Date
Oct 01, 2017
Volume
40
Issue
10
Pages
1395–1403
Identifiers
DOI: 10.1111/jfd.12617
PMID: 28383126
Source
Medline
Keywords
License
Unknown

Abstract

Diseases caused by motile aeromonads in freshwater fish have been generally assumed to be linked with mainly Aeromonas hydrophila while other species were probably overlooked. Here, we identified two isolates of non-A. hydrophila recovered from Nile tilapia exhibiting disease and mortality after exposed to transport-induced stress and subsequently confirmed their virulence in artificial infection. The bacterial isolates were identified as Aeromonas jandaei and Aeromonas veronii based on phenotypic features and homology of 16S rDNA. Experimental infection revealed that the high dose of A. jandaei (3.7 × 106 CFU fish-1 ) and A. veronii (8.9 × 106 CFU fish-1 ) killed 100% of experimental fish within 24 h, while a 10-fold reduction dose killed 70% and 50% of fish, respectively. When the challenge dose was reduced 100-fold, mortality of the fish exposed to A. jandaei and A. veronii decreased to 20% and 10%, respectively. The survivors from the latter dose administration were rechallenged with respective bacterial species. Lower mortality of rechallenged fish (0%-12.5%) compared to the control groups receiving a primary infection (37.5%) suggested that the survivors after primary infection were able to resist secondary infection. Fish exposed to either A. jandaei or A. veronii exhibited similar clinical signs and histological manifestation.

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