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Enzymatic dehydrogenation of cyclo(l-Phe–l-Leu) to a bioactive derivative, albonoursin1 Dedicated to Professor Hideaki Yamada in honor of his 70th birthday.1

Authors
Journal
Journal of Molecular Catalysis B Enzymatic
1381-1177
Publisher
Elsevier
Publication Date
Volume
6
Issue
3
Identifiers
DOI: 10.1016/s1381-1177(98)00079-4
Keywords
  • Bioconversion
  • Dehydrodiketopiprazines
  • Cyclic Dipeptide
  • Dioxopiperazine
  • Piprazinedione
Disciplines
  • Physics

Abstract

Abstract The cell-free extract of Streptomyces sp. KO-2388, an albonoursin-producing strain, was found to catalyze the conversion of cyclo( l-Phe– l-Leu) to albonoursin. The conversion activity was simply determined by measuring the increase in ultraviolet (UV) absorption of the reaction mixture at 317 nm, λ max ( ε 25,400) of albonoursin, where cyclo( l-Phe– l-Leu) had no absorption. The optimum pH and temperature of this bioconversion using the cell-free extract were determined to be pH 8.0–9.5 and 60°C, respectively. Under the optimum conditions, 620 mg/l of albonoursin was obtained with a conversion ratio of 62% after 24 h incubation.

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