The conodont assemblages of three stratigraphic sections from lower Serpukhovian rocks of the Guadiato Area (southwestern Spain) are composed of fifteen taxa, included in nine genera. Many of them are identified in open nomenclature due to the poor preservation of the specimens, of which most of them are fragmented, the presence of juvenile specimens, and the current controversy in the recognition of some multielement apparatuses. Generally, the faunas are not highly diversified and there is a low abundance of specimens in the horizons that yield conodonts. The assemblages include: Cavusgnathus navicula, Cavusgnathus cf. navicula, Gnathodus girtyi, of which two subspecies were identified, Gnathodus girtyi girtyi and Gnathodus girtyi meischneri, Hindeodontoides? sp., Hindeodus cristula, Idioprioniodus healdi, Idioprioniodus spp., Kladognathus macrodentata?, Kladognathus tenuis-complectens, Lochriea? sp., Mestognathus bipluti, Synclydognathus geminus, Synclydognathus spp. and many unidentifiable fragments.