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Characterization of the precursor form of the exocellular levansucrase fromBacillus subtilis

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
119
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(84)80320-4
Disciplines
  • Biology

Abstract

Summary Expression of the cloned levansucrase gene ( sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacry-lamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH 2 terminal region of the protein.

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