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Radiation inactivation analysis of fusion and hemolysis by vesicular stomatitis virus

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
163
Issue
2
Identifiers
DOI: 10.1016/0042-6822(88)90304-2
Disciplines
  • Biology

Abstract

Abstract Radiation inactivation analysis was used to determine the size of the functional unit responsible for fusion of vesicular stomatitis virus (VSV) with cardiolipin or phosphatidylcholine-phosphatidylethanolamine (1:1) liposomes, and for VSV-induced hemolysis. When radiation-insensitive background values were subtracted, the calculated functional units for all three activities were similar, ranging from 866 to 957 kDa, equivalent to about 15 G protein molecules. This is in is iking contrast to results of similar studies with influenza and Sendai viruses, in which the functional unit corresponded in size to a single fusion protein monomer, and suggests that VSV fusion may occur by a different mechanism.

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