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DNA polymerase I: from crystal structure to function via genetics

Authors
Journal
Trends in Biochemical Sciences
0968-0004
Publisher
Elsevier
Publication Date
Volume
12
Identifiers
DOI: 10.1016/0968-0004(87)90143-5
Disciplines
  • Medicine

Abstract

Abstract The Klenow fragment of E. coli DNA polymerase I has two separate structural domains. The polymerase active site is located on the larger C-terminal domain, about 30 Å from the 3′–5′ exonuclease active site on the N-terminal domain. We are using combination of X-ray crystallography and molecular genetics to investigate the mechanisms of both reactions and the way that these two distant active sites can work together to enhance the fidelity of DNA synthesis.

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