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Molecular weight, structure and shape of oat (1→3),(1→4)-β-d-glucan fractions obtained by enzymatic degradation with (1→4)-β-d-glucan 4-glucanohydrolase fromTrichodermareesei

Authors
Journal
Carbohydrate Polymers
0144-8617
Publisher
Elsevier
Publication Date
Volume
46
Issue
3
Identifiers
DOI: 10.1016/s0144-8617(00)00329-5
Keywords
  • Mixed-Linkage (1→3)
  • (1→4)-β-D-Glucan
  • Oats
  • Cellulase
  • Molecular Weight
  • Shape
  • Structure
  • Kinetics
Disciplines
  • Biology

Abstract

Abstract Oat β-glucan was partially degraded with (1→4)-β- d-glucan 4-glucanohydrolase for different periods of time. Weight average molecular weight and weight average intrinsic viscosity were obtained from SEC-RI-RALLS-Visc and ranged from 2200 to 213,900 g/mol and 7 to 316 ml/g, respectively. The viscosity equation determined [ η ̄ ] w =1.06×10 −2 M ̄ w 0.86 indicated an extended random coil conformation. When the coil was modelled as a worm-like chain a persistent length of 3.65 nm was obtained. The hydrolysis products identified after extensive degradation were glucose, cellobiose, laminaribiose, 4- O-β-laminaribiosyl d-glucose, 4- O-β-laminaribiosyl d-cellobiose and 3- O-β-cellobiosyl d-cellobiose showing that other enzyme activities were present. The depolymerization kinetics suggested that longer sequences of consecutive (1→4)-linkages represent fast hydrolysable sites and the presence of higher relative proportions of (1→3)-linkages restricts the affinity of the enzyme.

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