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[Advances in two-photon imaging technology].

Authors
  • Xia, Weiqiang
  • Zhou, Yuan
  • Shi, Ming
Type
Published Article
Journal
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation
Publication Date
May 01, 2011
Volume
35
Issue
3
Pages
204–208
Identifiers
PMID: 21954580
Source
Medline
License
Unknown

Abstract

As a new kind of advanced nonlinear imaging approach, two-photon fluorescence microscopy technology is wildly used in the field of live cell and tissue imaging, especially focusing on long-term dynamic three-dimensional cell imaging. This paper firstly presents the principle and characteristic of two-photon fluorescence microscopy. Then, the paper focuses on the three key aspects of the viability of the specimen, sensitivity of detection, as well as the speed of acquisition. In the end, the future prospect of development and application of two-phonon imaging technology are predicted.

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