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Adsorbed solution model for prediction of normal-phase chromatography process with varying composition of the mobile phase.

Authors
  • Piatkowski, Wojciech
  • Petrushka, Igor
  • Antos, Dorota
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Oct 21, 2005
Volume
1092
Issue
1
Pages
65–75
Identifiers
PMID: 16188561
Source
Medline
License
Unknown

Abstract

The adsorbed solution model has been used to predict competitive adsorption equilibria of the solute and the active component of mobile phase in a normal-phase liquid chromatography system. The inputs to the calculations were the single adsorption isotherms accounting for energetic heterogeneity of the adsorbent surface and non-ideality of the mobile phase solution. The competitive adsorption model has been coupled with a model of the column dynamics and used for simulating of chromatography process at different mobile phase composition. The predictions have been verified by comparing the simulated and experimental chromatograms. The model allowed quantitative prediction of chromatography process on the basis of the pure-species adsorption isotherms.

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