Affordable Access

Adh locus in maize for detection of mutagens in the environment.

Authors
Publication Date
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

The advantages of the maize Adh system for low level mutagenesis studies are discussed. They include high mutation rate and the availability of both a strong selection system for null mutants and a sensitive method for detection of variants. The null selection involves treatment of pollen with allyl alcohol which is converted by the Adh enzyme to the toxic acrylaldehyde. Adh variants can be detected by alteration in affinity for phytic acid as well as thermal stability and electrophoretic mobility.

Statistics

Seen <100 times