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Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure

Journal
PLoS Biology
1544-9173
Publisher
Public Library of Science
Publication Date
Volume
7
Issue
5
Identifiers
DOI: 10.1371/journal.pbio.1000102
Disciplines
  • Mathematics

Abstract

The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA′/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble. © 2009 Korkhin et al.

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