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Adenosine deaminase activity in pig saliva: analytical validation of two spectrophotometric assays.

Authors
  • Tecles, Fernando1, 2
  • Rubio, Camila P1, 2
  • Contreras-Aguilar, María D1, 2
  • López-Arjona, Marina1, 2
  • Martínez-Miró, Silvia1, 2
  • Martínez-Subiela, Silvia1, 2
  • Cerón, José J1, 2
  • 1 Departments of Animal Medicine and Surgery (Tecles, Rubio, Contreras-Aguilar, Martínez-Subiela, Cerón), Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, Spain. , (Spain)
  • 2 Animal Production (Martínez-Miró), Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, Spain. , (Spain)
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Nov 01, 2017
Identifiers
DOI: 10.1177/1040638717742947
PMID: 29145785
Source
Medline
Keywords
License
Unknown

Abstract

We validated 2 assays for the measurement of adenosine deaminase (ADA) activity in the saliva of pigs: the Giusti-Galanti manual method (ADA-GG) and a commercial automated assay (Diazyme Laboratories; ADA-D). Intra-assay coefficients of variation (CVs) were <7 and 9%, and interassay CVs were <12 and 5%, for ADA-GG and ADA-D, respectively. Accuracy was measured by 2 methods: recovery and linearity-under-dilution. Recovery was 82.4-106.8% for ADA-GG, and 92.8-107.9% for ADA-D. Serial dilutions showed R2 > 0.95 and 0.99 for ADA-GG and ADA-D, respectively. Linear regression between the methods gave R2 = 0.997 ( p < 0.0001), and a Bland-Altman plot showed a proportional bias of 112 IU/L (95% confidence interval of -99 to 322 IU/L) for ADA-D. No significant differences were observed between the results obtained by either method in saliva or serum. ADA activity was much higher in porcine saliva than in serum. Salivary ADA activity was significantly higher in lame pigs compared to healthy animals. However, serum ADA activity was significantly lower in lame pigs.

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