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Screening and Design of Immobilized Biocatalysts by Means of Kinetic Characterization on Enzyme Thermistor/Thermal Assay Probe

Elsevier B.V.
DOI: 10.1016/s1569-2558(08)60124-1
  • Biology
  • Design
  • Mathematics


Abstract Screening and design of immobilized biocatalysts (IMB) is a time-consuming process; ideally, the process should be universal, fast, convenient, precise, and sufficiently reproducible. These requirements are met by enzymic flow (micro)calorimetry (EFMC) also known as “enzyme thermistor” or “thermal assay probe.” Adaptation of EFMC to real measurements of the reaction rates requires coupling of the mathematical description of the reaction-diffusion phenomena in the EFMC column with heat balance and, subsequently, experimental verification of the mathematical model. This paper presents a brief review of the results obtained during the adaptation of EFMC for the characterization of the microkinetic properties of IMB and their further application for screening and design of IMB.

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