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Effect ofBrn-3adeficiency on parvalbumin-immunoreactive primary sensory neurons in the dorsal root ganglion

Authors
Journal
Developmental Brain Research
0165-3806
Publisher
Elsevier
Publication Date
Volume
150
Issue
1
Identifiers
DOI: 10.1016/j.devbrainres.2004.02.004
Keywords
  • Transcription Factor
  • Knockout Mouse
  • Parvalbumin
  • Proprioceptor
  • Immunohistochemistry
Disciplines
  • Chemistry

Abstract

Abstract Immunohistochemistry for parvalbumin, a marker for primary proprioceptors, was performed on the dorsal root ganglion (DRG) of wildtype and knockout mice for Brn-3a at postnatal day 0 and embryonic day 18.5. The DRG contained many parvalbumin-immunoreactive (ir) neurons in wildtype (5.4%) and knockout mice (5.6%). Cell size analysis demonstrated that such neurons were mostly medium-sized to large in these mice. Therefore, it is unlikely that the survival of proprioceptors is dependent upon Brn-3a in the DRG. In the dorsal column and gray matter of the spinal cord of knockout mice, however, parvalbumin-ir nerve fibers were sparse compared to wildtype mice. The number of parvalbumin-ir varicosities around motoneurons decreased in the mutant. Thus, our data suggest that Brn-3a may play an important role in the central projection and terminal formation of DRG proprioceptors in the spinal cord.

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