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Adapter reagents for protein site specific dye labeling.

Authors
Type
Published Article
Journal
Biopolymers
Publisher
Wiley (John Wiley & Sons)
Volume
102
Issue
3
Pages
273–279
Identifiers
DOI: 10.1002/bip.22481
Source
UCSC Aging biomedical-ucsc
License
Unknown

Abstract

Chemoselective protein labeling remains a significant challenge in chemical biology. Although many selective labeling chemistries have been reported, the practicalities of matching the reaction with appropriately functionalized proteins and labeling reagents is often a challenge. For example, we encountered the challenge of site specifically labeling the cellular form of the murine Prion protein with a fluorescent dye. To facilitate this labeling, a protein was expressed with site specific p-acetylphenylalanine. However, the utility of this acetophenone reactive group is hampered by the severe lack of commercially available aminooxy fluorophores. Here we outline a general strategy for the efficient solid phase synthesis of adapter reagents capable of converting maleimido-labels into aminooxy or azide functional groups that can be further tuned for desired length or solubility properties. The utility of the adapter strategy is demonstrated in the context of fluorescent labeling of the murine Prion protein through an adapted aminooxy-Alexa dye.

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