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Temperature-dependent retention of a tryptophan-operon-bearing plasmid inEscherichia coli

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
31
Identifiers
DOI: 10.1016/0378-1119(84)90201-4
Keywords
  • Plasmid Maintenance
  • Pbr322 Derivative
  • Valyl-Trna Synthetase
  • Recombinant Dna
  • Stable Cloning Vector
Disciplines
  • Biology

Abstract

Abstract Derivatives of plasmid pBR322 carrying the tryptophan operon are rapidly lost from a growing culture. The valS gene from Escherichia coli was combined with the tryptophan operon on a pBR322 derivative, pSGS21. In a thermosensitive valS mutant of E. coli K-12 host, plasmid pSGS21 was stably inherited for over 200 generations at temperatures nonpermissive for the chromosomal mutation. At 30 °C, a temperature at which the host valS tsprotein is functional, the plasmid was lost at a rate of 1.2% per cell generation.

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