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Acute oxidative stress affects IL-8 and TNF-α expression in IPEC-J2 porcine epithelial cells.

Authors
  • Paszti-Gere, Erzsebet
  • Csibrik-Nemeth, Edina
  • Szeker, Krisztina
  • Csizinszky, Rita
  • Jakab, Csaba
  • Galfi, Peter
Type
Published Article
Journal
Inflammation
Publisher
Springer-Verlag
Publication Date
Jun 01, 2012
Volume
35
Issue
3
Pages
994–1004
Identifiers
DOI: 10.1007/s10753-011-9403-8
PMID: 22083491
Source
Medline
License
Unknown

Abstract

Reactive oxygen species are implicated in cell and tissue damage in a number of diseases including acute and chronic inflammation of the gut. Effects of H(2)O(2) exposure on non-carcinogenic porcine epithelial cell line, IPEC-J2 cells cultured on collagen-coated membrane inserts were monitored based on transepithelial electrical resistance (TER) change, extent of necrotic cell damage, gene expression of inflammatory cytokines IL-8 and TNF-α. Furthermore, the junction proteins claudin-1 and E-cadherin were also investigated by immunohistochemistry. Peroxide (1mM) increased IL-8 and TNF-α gene expression levels significantly allowing 1 h recovery time without affecting the cellular distribution of junction proteins, TER and cell survival rate. In conclusion, the IPEC-J2 cell line on membrane insert was introduced as a fast and reliable investigation tool for oxidative stimuli-triggered intestinal inflammation and in the future as a screening method for antioxidant and probiotic candidates.

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