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Acute kidney injury induced by glycerol is worsened by orchiectomy and attenuated by testosterone replacement.

  • de Souza, Samira Itana1
  • Rocha, Edilene Cândido1
  • Ferraz, Halanna Rocha1
  • Dias, Jéssica Afonso1
  • Seguro, Antonio Carlos2
  • Volpini, Rildo Aparecido2
  • Canale, Daniele2
  • de Bragança, Ana Carolina2
  • Shimizu, Maria Heloisa Massola2
  • Marques, Lucas Miranda1
  • de Magalhães, Amélia Cristina Mendes1
  • Coimbra, Terezila Machado3
  • de Jesus Soares, Telma4
  • 1 Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Bahia 45029-094, Brazil. , (Brazil)
  • 2 Laboratório de Investigação Médica 12 (LIM12), Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. , (Brazil)
  • 3 Departamento de Fisiologia, Universidade de São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil. , (Brazil)
  • 4 Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Bahia 45029-094, Brazil. Electronic address: [email protected] , (Brazil)
Published Article
Publication Date
Nov 07, 2020
DOI: 10.1016/j.steroids.2020.108755
PMID: 33171132


Although several studies have demonstrated that the male gender represents an independent risk factor for renal disease, evidence shows that androgens exert renal protective actions. The findings are controversial and no studies have evaluated the effects of orchiectomy and testosterone replacement on glycerol-induced renal injury. Male Wistar rats were submitted to orchiectomy or sham surgery and divided into four groups: SC, sham control rats injected with NaCl; SG, sham rats injected with glycerol; OG, orchiectomized rats injected with glycerol; OGT, orchiectomized rats injected with glycerol and testosterone. Testosterone was administered daily for 14 days in the OGT group. After 11 days of testosterone replacement in the OGT group, SC rats were submitted to a saline injection, while SG, OG and OGT rats received glycerol. All rats were euthanized three days after injections. OG rats presented higher serum creatinine and urea, and sodium excretion, compared to SC and SG, while testosterone attenuated these changes. Acute tubular necrosis was also mitigated by testosterone. Renal immunostaining for macrophages, lymphocytes and NF-κB was higher in OG compared to SC and SG. In addition, renal interleukin-1β, Caspase 3 and AT1 gene expression was higher in OG rats compared to SG. Testosterone attenuated these alterations, except the NF-κB immunostaining. The renal NO was lower in OG rats compared to SG. Only the OG rats presented decreases in serum NO and renal HO-1, and increased TNF-α, angiotensinogen and AT1 expression compared to SC. We conclude that orchiectomy worsened glycerol-induced kidney injury, while testosterone attenuated this renal damage. Copyright © 2020. Published by Elsevier Inc.

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