Affordable Access

deepdyve-link
Publisher Website

Acute invasive fungal rhinosinusitis: Molecular identification and update in management of frozen section biopsy.

Authors
  • Shamsaei, Sina1
  • Falahati, Mehraban2
  • Farahyar, Shirin3
  • Raiesi, Omid4
  • Haghighi, Leila1
  • Farahani, Hamed Eraghiye5
  • Akhavan, Asghar6
  • Shamsaie, Alireza7
  • Yarahmadi, Mohammad8
  • Keymaram, Mahyar1
  • 1 Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 2 Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: [email protected] , (Iran)
  • 3 Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Microbial Biotechnology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 4 Department of Parasitology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran. , (Iran)
  • 5 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 6 Department of Otorhinolaryngology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. , (Iran)
  • 7 Department of Pathology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. , (Iran)
  • 8 Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. , (Iran)
Type
Published Article
Journal
Microbial Pathogenesis
Publisher
Elsevier
Publication Date
Aug 06, 2021
Pages
105125–105125
Identifiers
DOI: 10.1016/j.micpath.2021.105125
PMID: 34371109
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The clinical diagnosis of Acute Invasive Fungal Rhinosinusitis (AIFRS) is technically difficult because it presents with non-exclusive and nonspecific clinical symptoms. Laboratory confirmation (usually via histopathologic techniques such as formalin-fixed paraffin-embedded (FFPE)) is necessary but it is time-consuming, despite the urgent need for timely diagnosis of AIFRS for effective management. This study aimed to investigate the sensitivity and specificity of the GMS frozen-section biopsy in the diagnosis of AIFRS and compare the same with that of different tissue staining methods to provide valid decision-grounds that may guide clinicians in prompt diagnosis of acute fungal invasive rhinosinusitis. A cross-sectional study was conducted in the Medical Mycology Laboratory, Faculty of Medicine, Iran University of Medical Sciences between 2018 and 2020 on 200 patients with suspected AIFRS referred to Baqiyatallah and Imam Khomeini Hospital, Tehran. All patients were subjected to diagnostic nasal endoscopy and computed tomography (CT) scan of paranasal sinuses. Magnetic resonance imaging (MRI) was done in cases of suspected intracranial extension. After screening by routine mycological examination, the diagnosis was confirmed using complementary molecular methods. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the frozen-section biopsy were also compared with FFPE. Of the 200 suspect patients, 47 cases (23.5%) met the criteria for AIFRS. Species of the genus Aspergillus were the predominant 27 (57.4%) followed by Mucorales species 10 (21.3%), and Fusarium spp 3 (6.4%). Also, 3 cases (6.4%) of co-infection due to Aspergillus/Rhizopus were reported. The accuracy, sensitivity, specificity, PPV, and NPV of frozen section assessments were 99.5%, 97.9%, 100%, 100% and 99.3%, respectively. For GMS frozen-section alone, sensitivity, specificity, NPV, and PPV was 100%. Overall, the calculated accuracy of FFPE was 98.5%, sensitivity was 94%, specificity was 100%, PPV was 100%, and NPV was 98.1%. Examination of the frozen-section biopsy is a highly predictive tool for a rapid and effective diagnosis of patients with suspected AIFRS. We observed that GMS frozen-section is a fast and reliable exam to confirm the diagnosis of fungal invasion, with good accuracy, sensitivity, and specificity compared to the gold-standard FFPE biopsy. Copyright © 2021. Published by Elsevier Ltd.

Report this publication

Statistics

Seen <100 times