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Acute heat-treatment disrupts inhibin-related protein production and gene expression in the adult rat testis.

Authors
  • Aldahhan, Rashid A1
  • Stanton, Peter G2
  • Ludlow, Helen3
  • de Kretser, David M2
  • Hedger, Mark P2
  • 1 Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Victoria, Australia; Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, Australia; Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia. Electronic address: [email protected] , (Australia)
  • 2 Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Victoria, Australia; Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria, Australia. , (Australia)
  • 3 Oxford Brookes University, Oxford, UK.
Type
Published Article
Journal
Molecular and cellular endocrinology
Publication Date
Dec 01, 2019
Volume
498
Pages
110546–110546
Identifiers
DOI: 10.1016/j.mce.2019.110546
PMID: 31422101
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Heat reversibly disrupts spermatogenesis, but the effects on Sertoli cell (SC) function and inhibin/activin-related proteins are less well-defined. Adult rat testis weights decreased by 40% within 2 weeks after heat-treatment (43 °C, 15 min), due to loss of pachytene spermatocytes and round spermatids. Coincident effects were reduced SC nuclear volume at one week and >50% reduction in expression of several critical SC genes (Inha, Cld11, Gja1, Tjp1, Cldn3) by 2 weeks. Leydig cell steroidogenic enzymes, Cyp11a1, Hsd3b1, were also reduced. Activin gene expression was unaffected at this time, but expression of the activin-binding protein, follistatin (Fst), increased >2-fold. At 4-8 weeks, coincident with the recovery of spermatocytes and early spermatids, but progressive loss of elongated spermatids, most SC genes had recovered; however, testicular activin A was reduced and activin B increased. At 8 weeks, serum inhibin was decreased and, consequently, serum FSH increased. Crucially, germ cell damage was not associated with a significant inflammatory response. At 14 weeks, most testicular parameters had returned to normal, but testis weights remained slightly reduced. These data indicate that, following acute heat-treatment, expression of several key Sertoli and Leydig cell genes declined in parallel with the initial loss of meiotic germ cells, whereas activins were responsive to the subsequent loss of mature spermatids, leading to an increase in testicular activin B production relative to activin A. Copyright © 2019 Elsevier B.V. All rights reserved.

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