Chronic inflammation contributes to the pathophysiology of many chronic diseases. Dietary fibers and antioxidants may exert anti-inflammatory effects. The objective of this study was to determine whether meals with different fibers and antioxidants may elicit a different response in inflammatory markers in healthy volunteers. On 3 separate days, subjects (n = 8) consumed one of three isocaloric meals with different antioxidant (vitamin E, selenium and β-carotene) and fiber content (high, intermediate and low) in a randomized crossover design. Blood samples were collected at different times: 0 min (before the meal), and 30 and 240 min after the meal. Plasma interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α, glucose and insulin content were evaluated at each time point. There were no significant differences for any of the parameters at baseline. Furthermore, plasma levels of TNF-α, IL-6 and IL-10 were unchanged at the 30- and 240-min time points whatever meal consumed. Moreover, the cytokine responses to glucose and insulin intake were not significantly different between experimental conditions. In conclusion, isocaloric meals with different fiber, β-carotene, vitamin E and selenium contents do not acutely affect inflammatory markers in healthy young males.