Affordable Access

deepdyve-link
Publisher Website

Activity- and reactivity-based proteomics: Recent technological advances and applications in drug discovery.

Authors
  • Benns, Henry James1
  • Wincott, Ceire Joanne2
  • Tate, Edward William3
  • Child, Matthew Andrew4
  • 1 Department of Life Sciences, London, UK; Department of Chemistry, Imperial College London, London, UK.
  • 2 Department of Life Sciences, London, UK.
  • 3 Department of Chemistry, Imperial College London, London, UK.
  • 4 Department of Life Sciences, London, UK. Electronic address: [email protected]
Type
Published Article
Journal
Current opinion in chemical biology
Publication Date
Aug 05, 2020
Volume
60
Pages
20–29
Identifiers
DOI: 10.1016/j.cbpa.2020.06.011
PMID: 32768892
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Activity-based protein profiling (ABPP) is recognized as a powerful and versatile chemoproteomic technology in drug discovery. Central to ABPP is the use of activity-based probes to report the activity of specific enzymes or reactivity of amino acid types in complex biological systems. Over the last two decades, ABPP has facilitated the identification of new drug targets and discovery of lead compounds in human and infectious disease. Furthermore, as part of a sustained global effort to illuminate the druggable proteome, the repertoire of target classes addressable with activity-based probes has vastly expanded in recent years. Here, we provide an overview of ABPP and summarise the major technological advances with an emphasis on probe development. Copyright © 2020 Elsevier Ltd. All rights reserved.

Report this publication

Statistics

Seen <100 times