Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer

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Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer

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BioMed Central
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PMC
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  • Biology
  • Chemistry
  • Design
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Abstract

1471-2407-6-211.fm ral ss BioMed CentBMC Cancer Open AcceResearch article Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer Tae-Dong Kim2, Kyoung-Sub Song2, Ge Li1, Hoon Choi1, Hae-Duck Park1, Kyu Lim2,3, Byung-Doo Hwang2,3 and Wan-Hee Yoon*1,3 Address: 1Department of Surgery, College of Medicine, Chungnam National University and Hospital, Daejeon, 301-721, Korea, 2Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon, 301-721, Korea and 3Cancer Research Institute, Chungnam National University, Daejeon, Korea Email: Tae-Dong Kim - [email protected]; Kyoung-Sub Song - [email protected]; Ge Li - [email protected]; Hoon Choi - [email protected]; Hae-Duck Park - [email protected]; Kyu Lim - [email protected]; Byung- Doo Hwang - [email protected]; Wan-Hee Yoon* - [email protected] * Corresponding author Abstract Background: Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-type plasminogen activator (uPA) are involved in colorectal cancer invasion and metastasis. There is still debate whether the activity of MMP-2 and MMP-9 differs between tumors located in the colon and rectum. We designed this study to determine any differences in the expression of MMP-2, MMP-9 and uPA system between colon and rectal cancer tissues. Methods: Cancer tissue samples were obtained from colon carcinoma (n = 12) and rectal carcinomas (n = 10). MMP-2 and MMP-9 levels were examined using gelatin zymography and Western blotting; their endogenous inhibitors, tissue inhibitor of metalloproteinase-2 (TIMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed by Western blotting. uPA, uPAR and PAI-1 were examined using enzyme-linked immunosorbent assay (ELISA). The activity of uPA was assessed by casein-plasminogen zymography. Results: In both colon and rectal tumors, MMP-2, MMP-9 and TIMP-1 protein levels were higher than in correspond

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