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Activation of Wnt signaling in Axin2+ cells leads to osteodentin formation and cementum overgrowth.

Authors
  • Shi, Peilei1
  • Xie, Xudong1
  • Xu, Chunmei1
  • Wu, Yafei1
  • Wang, Jun1
  • 1 State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, Med-X Center for Materials, Sichuan University, Chengdu, China. , (China)
Type
Published Article
Journal
Oral diseases
Publication Date
Nov 01, 2023
Volume
29
Issue
8
Pages
3551–3558
Identifiers
DOI: 10.1111/odi.14472
PMID: 36520568
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this study, we used the mouse incisor model to investigate the regulatory mechanisms of Wnt/β-catenin signaling on Axin2+ cells in tooth development. Axin2lacZ/+ reporter mice were used to define the expression pattern of Axin2 in mouse incisors. We traced the fate of Axin2+ cells from postnatal Day 21 (P21) to P56 using Axin2CreERT2/+ and R26RtdTomato/+ reporter mice. For constitutive activation of Wnt signaling, Axin2CreERT2/+ , β-cateninflox(Ex3)/+ , and R26RtdTomato/+ (CA-β-cat) mice were generated to investigate the gain of function (GOF) of β-catenin in mouse incisor growth. The X-gal staining of Axin2lacZ/+ reporter mice and lineage tracing showed that Axin2 was widely expressed in dental mesenchyme of mouse incisors, and Axin2+ cells were essential cell sources for odontoblasts, pulp cells, and periodontal ligament cells. The constitutive activation of Wnt signaling in Axin2+ cells resulted in the formation of osteodentin featured with increased DMP1 and dispersed DSP expression and overgrowth of cementum. Wnt signaling plays a key role in the differentiation and maturation of Axin2+ cells in mouse incisors. © 2022 Wiley Periodicals LLC.

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