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Activation of the transcription factor NF-kappaB by Campylobacter jejuni.

Authors
  • Mellits, Kenneth H
  • Mullen, Joseph
  • Wand, Matthew
  • Armbruster, Gisèle
  • Patel, Amit
  • Connerton, Phillippa L
  • Skelly, Maeve
  • Connerton, Ian F
Type
Published Article
Journal
Microbiology (Reading, England)
Publication Date
Sep 01, 2002
Volume
148
Issue
Pt 9
Pages
2753–2763
Identifiers
PMID: 12213922
Source
Medline
License
Unknown

Abstract

Campylobacter jejuni is a food-borne pathogen responsible for infectious enterocolitis. The early-response transcription factor NF-kappa B triggers the expression of genes associated with cellular immune and inflammatory responses. Co-incubation of HeLa cells with viable C. jejuni leads to the activation of the transcription factor NF-kappa B as determined by specific induction of a cellular luciferase-based reporter. Boiled cell-free extracts of C. jejuni are also potent dose-dependent stimulators of NF-kappa B-dependent transcription, the levels of which can reach up to 1000-fold as compared with independent controls. Using both cultured HeLa cells and human colonic epithelial (HCA-7) cells, the activation of NF-kappa B by C. jejuni boiled extract has been monitored through the degradation of IKB alpha and DNA binding of the nuclear translocated p50/p65 heterodimer of NF-kappa B. These events are co-ordinated with elaboration of the pro-inflammatory cytokine interleukin-8. Fractionation of the boiled C. jejuni extract suggests that the majority of the bioactive component has a molecular mass of 3 kDa or less, which is insensitive to proteinase K treatment.

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