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Activation of synovial fibroblasts from patients at revision of their metal-on-metal total hip arthroplasty

Authors
  • Xu, Jing1, 2
  • Yang, Junyao2, 3
  • Chen, Jian1
  • Zhang, Xiaoli2
  • Wu, Yuanhao2
  • Hart, Alister4
  • Nyga, Agata5, 6
  • Shelton, Julia C.2
  • 1 School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China , Shanghai (China)
  • 2 Queen Mary University of London, London, UK , London (United Kingdom)
  • 3 Faculty of Life Science and Medicine, King’s College London, London, SE5 9NU, UK , London (United Kingdom)
  • 4 Royal National Orthopaedic Hospital, University College London, Stanmore, HA7 4AP, UK , Stanmore (United Kingdom)
  • 5 University College London, London, NW3 2QG, UK , London (United Kingdom)
  • 6 Current affiliation: MRC LMB, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge, CB2 0QH, UK , Cambridge (United Kingdom)
Type
Published Article
Journal
Particle and Fibre Toxicology
Publisher
BioMed Central
Publication Date
Aug 27, 2020
Volume
17
Issue
1
Identifiers
DOI: 10.1186/s12989-020-00374-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundThe toxicity of released metallic particles generated in metal-on-metal (MoM) total hip arthroplasty (THA) using cobalt chromium (CoCr) has raised concerns regarding their safety amongst both surgeons and the public. Soft tissue changes such as pseudotumours and metallosis have been widely observed following the use of these implants, which release metallic by-products due to both wear and corrosion. Although activated fibroblasts, the dominant cell type in soft tissues, have been linked to many diseases, the role of synovial fibroblasts in the adverse reactions caused by CoCr implants remains unknown. To investigate the influence of implants manufactured from CoCr, the periprosthetic synovial tissues and synovial fibroblasts from patients with failed MoM THA, undergoing a revision operation, were analysed and compared with samples from patients undergoing a primary hip replacement, in order to elucidate histological and cellular changes.ResultsPeriprosthetic tissue from patients with MoM implants was characterized by marked fibrotic changes, notably an increase in collagen content from less than 20% to 45–55%, an increase in α-smooth muscle actin positive cells from 4 to 9% as well as immune cells infiltration. Primary cell culture results demonstrated that MoM synovial fibroblasts have a decreased apoptosis rate from 14 to 6% compared to control synovial fibroblasts. In addition, synovial fibroblasts from MoM patients retained higher contractility and increased responsiveness to chemotaxis in matrix contraction. Their mechanical properties at a single cell level increased as observed by a 60% increase in contraction force and higher cell stiffness (3.3 kPa in MoM vs 2.18 kPa in control), as measured by traction force microscopy and atomic force microscopy. Further, fibroblasts from MoM patients promoted immune cell invasion by secreting monocyte chemoattractant protein 1 (MCP-1, CCL2) and induced monocyte differentiation, which could also be associated with excess accumulation of synovial macrophages.ConclusionSynovial fibroblasts exposed in vivo to MoM THA implants that release CoCr wear debris displayed dramatic phenotypic alteration and functional changes. These findings unravelled an unexpected effect of the CoCr alloy and demonstrated an important role of synovial fibroblasts in the undesired tissue reactions caused by MoM THAs.

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